Product Pathways - Lymphocyte Signaling
Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate) #11880
|11880S||100 µl (50 tests)||---||In Stock||---|
|11880||carrier free and custom formulation / quantity||email request|
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Applications Key: F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Mouse, Rat.
Specificity / Sensitivity
Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714.
HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).
HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.