Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate) #11880

Applications Reactivity Sensitivity Isotype
F H (M) (R) Endogenous Rabbit IgG

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM and hydrogen peroxide (green), using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate).

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714.

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).

HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).

  1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
  2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
  4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
  5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
  6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
  7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.
  8. Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
  9. Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
  10. Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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