Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

TGF-β Receptor II (D3A1) Rabbit mAb #11888

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 70-80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TGF-β Receptor II (D3A1) Rabbit mAb recognizes endogenous levels of total TGF-β receptor II protein. This antibody also cross-reacts with a protein of unknown origin at 120 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro107 of human TGF-β receptor II protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and HT-1080 cells using TGF-β Receptor II (D3A1) Rabbit mAb.

IP

IP

Immunoprecipitation of TGF-β receptor II from 293 cell extracts using Normal Rabbit IgG #2729 (lane 2) or TGF-β Receptor II (D3A1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TGF-β Receptor II (D3A1) Rabbit mAb.

Background

Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).

  1. Massague, J. et al. (2000) Cell 103, 295-309.
  2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
  3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
  4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
  5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
  6. López-Casillas, F. et al. (1991) Cell 67, 785-795.
  7. Wang, X.F. et al. (1991) Cell 67, 797-805.
  8. Derynck, R. et al. (1998) Cell 95, 737-740.
  9. Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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