Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CHD8 (D3C1) Rabbit mAb #11891

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 290 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CHD8 (D3C1) Rabbit mAb recognizes endogenous levels of total CHD8 protein. This antibody also cross-reacts with a protein of unknown origin at 140 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human CHD8 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from OVCAR8 and SR cells using CHD8 (D3C1) Rabbit mAb.

IP

IP

Immunoprecipitation of CHD8 from SH-SY5Y cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CHD8 (D3C1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CHD8 (D3C1) Rabbit mAb.

Background

CHD8 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by utilizing the energy derived from ATP hydrolysis to alter chromatin architecture (1,2). The nine CHD family members are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). In addition, CHD8 contains three CR (conserved region) domains, a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like domain, two BRK (brahma and kismet) domains, and a DNA-binding domain (2). The chromatin remodeling activity of CHD8 has been shown to be important for the regulation of a wide variety of genes, such as the HOX genes (5) and genes that are driven by β-catenin (6), p53 (7), estrogen receptor (8), or androgen receptor (9). CHD8 can also interact with the insulator binding protein CTCF and is required for CTCF insulator activity at multiple gene loci (10).

  1. Hargreaves, D.C. and Crabtree, G.R. (2011) Cell Res 21, 396-420.
  2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
  3. Delmas, V. et al. (1993) Proc Natl Acad Sci U S A 90, 2414-8.
  4. Woodage, T. et al. (1997) Proc Natl Acad Sci U S A 94, 11472-7.
  5. Yates, J.A. et al. (2010) FEBS Lett 584, 689-93.
  6. Thompson, B.A. et al. (2008) Mol Cell Biol 28, 3894-904.
  7. Nishiyama, M. et al. (2009) Nat Cell Biol 11, 172-82.
  8. Caldon, C.E. et al. (2009) Mol Cell Biol 29, 4623-39.
  9. Menon, T. et al. (2010) Mol Endocrinol 24, 1165-74.
  10. Ishihara, K. et al. (2006) Mol Cell 23, 733-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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