Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Ser216) Blocking Peptide #1190

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Description

This peptide is used to block Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 signal in immunohistochemistry.

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.

Background

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).

1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.

Application References

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Companion Products

• 4901 Phospho-cdc25C (Ser216) (63F9) Rabbit mAb