Cell Signaling Technology

Product Pathways - Metabolism

MDH2 (D8Q5S) Rabbit mAb #11908

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 35 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MDH2 (D8Q5S) Rabbit mAb recognizes endogenous levels of total MDH2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly306 of human MDH2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MDH2 (D8Q5S) Rabbit mAb.

Background

Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid cycle and malate/aspartate shuttle (1,2). MDH is widely expressed in organisms from most bacteria to all eukaryotes (2). The cytoplasmic MDH isoenzyme (cMDH or MDH1) primarily reduces oxaloacetate to malate in the malate/aspartate shuttle (1-3). The major function of the mitochondrial MDH isoenzyme (mMDH or MDH2) is to oxidize malate to oxaloacetate in the tricarboxylic acid cycle (1,2).

  1. Matsuda, T. et al. (2010) J Pharmacol Sci 112, 320-6.
  2. Minárik, P. et al. (2002) Gen Physiol Biophys 21, 257-65.
  3. Joh, T. et al. (1987) J Biol Chem 262, 15127-31.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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