Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

EWS Antibody #11910

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 85 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

EWS Antibody recognizes endogenous levels of total EWS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly142 of human EWS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using EWS Antibody.

IP

IP

Immunoprecipitation of EWS from HeLa cell extracts using Normal Rabbit IgG #2729 (lane 2) or EWS Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EWS Antibody.

Background

The Ewing sarcoma (EWS) protein is a member of the multifunctional FET (FUS, EWS, and TAF15) family of proteins (1,2). These proteins are RNA and DNA binding proteins that are thought to be important for both transcriptional regulation and RNA processing. EWS can be found as part of a fusion protein with various E-twenty six (ETS) family transcription factors, most commonly Fli-1, in the Ewing sarcoma family of tumors (1-4). The amino terminus of the EWS protein, containing the transcriptional activation domain, is fused to the DNA binding domain of the ETS transcription factor, causing aberrant expression of target genes (1-5). EWS interacts with the transcription initiation complex via TFIID and RNA polymerase II subunits, as well as transcriptional regulators, such as Brn3A and CBP/p300, which suggests a role for EWS in transcriptional regulation (1,6-9). EWS also interacts with multiple components of the splicing machinery, implicating a role for EWS in RNA processing (1,10-12). EWS regulates the expression of cyclin D1, which controls G1-S phase transition during the cell cycle, at the level of transcriptional activation and mRNA splicing. The EWS-Fli-1 fusion protein has been shown to promote the expression of the cyclin D1b splice variant in Ewing sarcoma cells (13). In addition, EWS regulates the DNA damage-induced alternative splicing of genes involved in DNA repair and stress response and is required for cell viability upon DNA damage (14). Consistent with these results, EWS knockout mice display hypersensitivity to ionizing radiation and premature cellular senescence, suggesting a role for EWS in homologous recombination and maintenance of genomic stability (15).

  1. Law, W.J. et al. (2006) Brief Funct Genomic Proteomic 5, 8-14.
  2. Kovar, H. (2011) Sarcoma 2011, 837474.
  3. Delattre, O. et al. (1992) Nature 359, 162-5.
  4. May, W.A. et al. (1993) Mol Cell Biol 13, 7393-8.
  5. Sorensen, P.H. et al. (1994) Nat Genet 6, 146-51.
  6. Bertolotti, A. et al. (1996) EMBO J 15, 5022-31.
  7. Bertolotti, A. et al. (1998) Mol Cell Biol 18, 1489-97.
  8. Araya, N. et al. (2003) J Biol Chem 278, 5427-32.
  9. Thomas, G.R. and Latchman, D.S. Cancer Biol Ther 1, 428-32.
  10. Chansky, H.A. et al. (2001) Cancer Res 61, 3586-90.
  11. Yang, L. et al. (2000) J Biol Chem 275, 37612-8.
  12. Knoop, L.L. and Baker, S.J. (2001) J Biol Chem 276, 22317-22.
  13. Sanchez, G. et al. (2008) Proc Natl Acad Sci U S A 105, 6004-9.
  14. Paronetto, M.P. et al. (2011) Mol Cell 43, 353-68.
  15. Li, H. et al. (2007) J Clin Invest 117, 1314-23.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products