Product Pathways - Transcription Factors
NFI-C Antibody #11911
PhosphoSitePlus® protein, site, and accession data: NFI-C
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 55-65 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 11911:
- Immunoprecipitation, Western Blotting
Specificity / Sensitivity
NFI-C Antibody recognizes endogenous levels of total NFI-C protein. This antibody also recognizes overexpressed NFI-B protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala263 of human NFI-C protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa and U-2 OS cells using NFI-C Antibody.
IP
Immunoprecipitation of NFI-C from PANC-1 cell extracts using Normal Rabbit IgG #2729 (lane 2) or NFI-C Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NFI-C Antibody.
Background
NFI-C belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). NFI-C is expressed in various tissues and regulates TGF-β dependent tooth development and hair follicle cycling (3-5). Research studies have shown that NFI-C directly represses FoxF1 transcription and suppresses the motility and invasiveness of breast cancer cells (6).
- Gronostajski, R.M. (2000) Gene 249, 31-45.
- Liang, B. et al. (2012) J Virol 86, 3264-75.
- Steele-Perkins, G. et al. (2003) Mol Cell Biol 23, 1075-84.
- Lee, D.S. et al. (2009) J Biol Chem 284, 17293-303.
- Plasari, G. et al. (2010) J Biol Chem 285, 34115-25.
- Nilsson, J. et al. (2010) Cancer Res 70, 2020-9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
