Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CHD4 (D8B12) Rabbit mAb #11912

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk (B) (Pg) (GP) (Hr) Endogenous 260 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Pg=Pig  GP=Guinea Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CHD4 (D8B12) Rabbit mAb recognizes endogenous levels of total CHD4 protein. Based on sequence alignment, this antibody is not predicted to cross-react with other CHD proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1391 of human CHD4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CHD4 (D8B12) Rabbit mAb.

IP

IP

Immunoprecipitation of CHD4 from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CHD4 (D8B12) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CHD4 (D8B12) Rabbit mAb.

Background

Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA-binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multiprotein complexes with their transcriptional activation or repression activity governed by other proteins within the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two plant homeodomain (PHD) zinc finger domains that bind directly to HDAC1 and HDAC2.

  1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
  2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
  3. Tong, J.K. et al. (1998) Nature 395, 917-21.
  4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
  5. Zhang, Y. et al. (1998) Cell 95, 279-89.
  6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
  7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
  8. Fujita, N. et al. (2003) Cell 113, 207-19.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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