Product Pathways - Metabolism
CAD Antibody #11933
PhosphoSitePlus® protein, site, and accession data: CAD
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 240 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 11933:
- Western Blotting
Specificity / Sensitivity
CAD Antibody recognizes endogenous levels of total CAD protein. This antibody may cross-react with a protein of unknown origin at ~45 kDa in some cell lines.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CAD protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from SW620, MEF, and C6 cells using CAD Antibody.
Background
CAD is essential for the de novo synthesis of pyrimidine nucleotides and possesses the following enzymatic activities: glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase. Thus, the enzyme converts glutamine to uridine monophosphate, a common precursor of all pyrimidine bases, and it is necessary for nucleic acid synthesis (1). In resting cells, CAD is localized mainly in the cytoplasm where it carries out pyrimidine synthesis. As proliferating cells enter S phase, MAP Kinase (Erk1/2) phosphorlyates CAD at Thr456, resulting in CAD translocation to the nucleus. As cells exit S phase, CAD is dephosphorylated at Thr456 and phosphorylated at Ser1406 by PKA, returning the pathway to basal activity (2). Various research studies have shown increased expression of CAD in several types of cancer, prompting the development of pharmacological inhibitors such as PALA. Further studies have identified CAD as a potential predictive early marker of prostate cancer relapse (3).
- Coleman, P.F. et al. (1977) J Biol Chem 252, 6379-85.
- Sigoillot, F.D. et al. (2005) J Biol Chem 280, 25611-20.
- Morin, A. et al. (2012) FASEB J 26, 460-7.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
