Cell Signaling Technology

Product Pathways - Neuroscience

Oligophrenin-1 Antibody #11939

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 92 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Oligophrenin-1 Antibody recognizes endogenous levels of total Oligophrenin-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp647 of human Oligophrenin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues and cell lines using Oligophrenin-1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Oligophrenin-1 (Oligophrenin-1-Myc; +), using Oligophrenin-1 Antibody.

Background

Oligophrenin-1 is a RhoGTPase-activating protein encoded by the gene OPHN1 (1). Oligophrenin-1 is composed of an N-terminal BAR domain, a pleckstrin homology domain, a central RhoGAP domain, and three putative C-terminal SH3-binding sites. Oligophrenin-1 plays a role in membrane signaling through interaction of its BAR domain with curved membranes, binding of its pleckstrin homology domain with membrane phosphoinositides, and interaction of the SH3-binding sites with adaptor proteins (1-3). Oligophrenin-1 regulates synaptic vesicle endocytosis (3) and plays an important role in dendritic spine morphogenesis (4). Furthermore, by interacting with the transcription factor Rev-erbα and protecting it from degradation, Oligophrenin-1 participates in the regulation of the circadian rhythm in the hippocampus (5). Research studies have demonstrated an involvement of Oligophrenin-1 in X-linked mental retardation (1).

  1. Billuart, P. et al. (1998) Nature 392, 923-6.
  2. Khelfaoui, M. et al. (2007) J Neurosci 27, 9439-50.
  3. Nakano-Kobayashi, A. et al. (2009) Curr Biol 19, 1133-9.
  4. Govek, E.E. et al. (2004) Nat Neurosci 7, 364-72.
  5. Valnegri, P. et al. (2011) Nat Neurosci 14, 1293-301.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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