Cell Signaling Technology

Product Pathways - Transcription Factors

OTX2 (D7Y3J) Rabbit mAb #11943

Applications Reactivity Sensitivity MW (kDa) Isotype
W H (M) (R) (Mk) (C) (B) (GP) (Hr) Endogenous 31, 33 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  B=Bovine  GP=Guinea Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

OTX2 (D7Y3J) Rabbit mAb recognizes endogenous levels of total OTX2 protein. This antibody recognizes both known human isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn251 of human OTX2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Y79, D283 Med, and WERI-Rb-1 cells using OTX2 (D7Y3J) Rabbit mAb.

Background

Orthodenticle homeobox 2 (OTX2) belongs to the bicoid subfamily of paired-box, homeodomain-containing transcription factors. OTX2 is a critically important neuronal transcription factor that functions to regulate the expression of cell cycle genes controlling proliferation and differentiation of neural progenitor cells (1-3). In addition to its neuronal development functions, it has been reported that OTX2 can function in a non-cell autonomous manner to promote survival of damaged retinal ganglion cells (4). OTX2 has also been shown to influence the susceptibility of post-mitotic neurons to toxic insult or physiological stress (3). Notably, aberrant expression of OTX2 has been strongly linked with neuronal tumor development. For example, research studies have found OTX2 is overexpressed in many medulloblastoma cell lines, and both overexpression and gene amplification were reported in a subset of primary medulloblastomas (5). In vitro studies support these observations, as targeted alterations in OTX2 expression directly affected both proliferation and senescence of medulloblastoma cell lines (6,7).

  1. Simeone, A. et al. (1993) EMBO J 12, 2735-47.
  2. Omodei, D. et al. (2008) Development 135, 3459-70.
  3. Chung, C.Y. et al. (2010) Brain 133, 2022-31.
  4. Torero Ibad, R. et al. (2011) J Neurosci 31, 5495-503.
  5. Adamson, D.C. et al. (2010) Cancer Res 70, 181-91.
  6. Bunt, J. et al. (2011) Int J Cancer 131, E21-32.
  7. Bunt, J. et al. (2010) Mol Cancer Res 8, 1344-57.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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