Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Metabolism

DLST (D22B1) XP® Rabbit mAb #11954

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H Endogenous 50 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DLST (D22B1) XP® Rabbit mAb recognizes endogenous levels of total DLST protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro188 of human DLST protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DLST (D22B1) XP® Rabbit mAb.

IP

IP

Immunoprecipitation of DLST from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DLST (D22B1) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DLST (D22B1) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using DLST (D22B1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

α-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle (1). Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex (2). Reduction of DLST increases reactive oxygen species production, suggesting its role in oxidative stress (2). Research has shown that deficiency of DLST in mice is linked to increased oxidative stress in mitochondria, a process that may be involved in the pathogenesis of Alzheimer's disease (2).

  1. Ohta, S. and Ohsawa, I. (2006) J Alzheimers Dis 9, 155-66.
  2. Dumont, M. et al. (2009) Free Radic Biol Med 47, 1019-27.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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