Product Pathways - TGF-beta/Smad Signaling
PhosphoPlus® Smad2 (Ser465/467) Antibody Duet #11958
|Duet Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108||100 µl||W||H M R Mi||60||Rabbit IgG|
|Smad2 (D43B4) XP® Rabbit mAb #5339||100 µl||W IP IF-IC F ChIP||H M R Mk||60||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Mi=Mink
Species in parentheses are predicted to react based on 100% sequence homology.
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).
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For Research Use Only. Not For Use In Diagnostic Procedures.