Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

LIN28B (D4H1) Rabbit mAb #11965

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 32, 21 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LIN28B (D4H1) Rabbit mAb recognizes endogenous levels of total LIN28B protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LIN28B protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA-2, Hep G2, and Huh7 cells using LIN28B (D4H1) Rabbit mAb.

IP

IP

Immunoprecipitation of LIN28B from NCCIT cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or LIN28B (D4H1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using LIN28B (D4H1) Rabbit mAb.

Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

  1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
  2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
  3. Cho, W.C. (2007) Mol Cancer 6, 60.
  4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
  5. West, J.A. et al. (2009) Nature 460, 909-13.
  6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
  7. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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