Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CDK12 Antibody #11973

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 205 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CDK12 Antibody recognizes endogenous levels of total CDK12 protein. This antibody also cross-reacts with proteins of unknown origin at 48 and 53 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val492 of human CDK12 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CDK12 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CDK12 isoform 2 (hCDK12-Myc/DDK; +), using CDK12 Antibody.

IP

IP

Immunoprecipitation of CDK12 from PC-3 cell extracts using Normal Rabbit IgG #2729 (lane 2) or CDK12 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CDK12 Antibody.


Background

Cyclin-dependent kinase 12 (CDK12/CRKRS/CRK7) is composed of a central CTD kinase domain, several proline-rich regions, and several amino-terminal arginine/serine (RS) motifs common to splicing factors (1). CDK12 is ubiquitously expressed and forms a complex with cyclin K that regulates phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (1-4). CDK12 is important for expression of a subset of long genes with high numbers of exons including some regulators of the DNA damage response, such as breast and ovarian cancer type 1 susceptibility protein 1 (BRCA1) and ataxia telangiectasia and Rad3-related (ATR) (3). Depletion of CDK12 results in spontaneous DNA damage and increased sensitivity to DNA damage agents (3). Research studies have shown that CDK12 is recurrently mutated in high-grade ovarian cancer (5,6). In addition, high levels of CDK12 are required to maintain pluripotency of embryonic stem cells (7).

  1. Ko, T.K. et al. (2001) J Cell Sci 114, 2591-603.
  2. Bartkowiak, B. et al. (2010) Genes Dev 24, 2303-16.
  3. Blazek, D. et al. (2011) Genes Dev 25, 2158-72.
  4. Cheng, S.W. et al. (2012) Mol Cell Biol 32, 4691-704.
  5. The Cancer Genome Atlas Research Network (2011) Nature 474, 609-15.
  6. Carter, S.L. et al. (2012) Nat Biotechnol 30, 413-21.
  7. Dai, Q. et al. (2012) J Biol Chem 287, 25344-52.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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