Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Tensin 2 Antibody #11990

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 145-155 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Tensin 2 Antibody recognizes endogenous levels of total Tensin 2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg155 of human Tensin 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Tensin 2 Antibody.

Background

Tensin 2 belongs to the Tensin family of cytoskeletal proteins that includes Tensin 1-3 and Cten, which couple integrins to the actin cytoskeleton (1). Tensin proteins contain SH2 and phosphotyrosine binding (PTB) domains, which enable interaction with diverse signaling molecules and proteins. Tensin family proteins play important roles in signal transduction, cell proliferation, and motility (2-5).Tensin 2 is localized to focal adhesions of various tissues with highest expression in the heart, kidney, and liver (6,7). Tensin 2 inhibits Akt/PKB signaling via a phosphatase tensin-type domain (8). However, Tensin 2 also mediates thrombopoietin/c-Mpl signaling, which promotes Akt signaling (9). Interaction with Tensin 2 is essential for the tumor suppressor function of Deleted in Cancer 1 (DLC1) (10-12).

  1. Lo, S.H. et al. (1994) Bioessays 16, 817-23.
  2. Lo, S.H. et al. (1994) J Cell Biol 125, 1067-75.
  3. Chen, H. and Lo, S.H. (2003) Biochem J 370, 1039-45.
  4. Katz, M. et al. (2007) Nat Cell Biol 9, 961-9.
  5. Chen, H. et al. (2002) Proc Natl Acad Sci U S A 99, 733-8.
  6. Clark, K. et al. (2010) J Cell Biochem 109, 808-17.
  7. Hafizi, S. et al. (2002) Biochem Biophys Res Commun 299, 793-800.
  8. Hafizi, S. et al. (2005) FASEB J 19, 971-3.
  9. Jung, A.S. et al. (2011) Cell Cycle 10, 1838-44.
  10. Chan, L.K. et al. (2009) PLoS One 4, e5572.
  11. Clark, K. et al. (2010) J Cell Biochem 109, 808-17.
  12. Chen, L. et al. (2012) J Biol Chem 287, 26104-14.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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