Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Calumenin (4C6) Mouse mAb #11991

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk Endogenous 47 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Calumenin (4C6) Mouse mAb recognizes endogenous levels of total calumenin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human calumenin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Calumenin (4C6) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Calumenin (4C6) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Calumenin belongs to the CREC family of proteins that also contains reticulocalbin, ERC-55/TCBP-49/E6BP, Cab45, and crocalbin/CBP-50. These EF-hand proteins localize to the endoplasmic reticulum (ER) and are involved in the secretory pathway in mammalian cells (1). Calumenin exists as two isoforms corresponding to alternativly spliced variants of the CALU gene (2). Calumenin has been shown to localize to the ER via a carboxy terminal HDEF ER retention signal (3), and undergoes secretion after trafficking through the secretory pathway (4). Secreted calumenin plays a role in amyloidosis by interacting with serum amyloid P component (5). Calumenin is also a chaperone for mutants of the cystic fibrosis transmembrane conductance regulator (CFTR) (6). Researchers performing a proteomics analysis of endometrial cancer identified calumenin as a potential target for endometrial carcinoma (7).

  1. Honoré, B. and Vorum, H. (2000) FEBS Lett 466, 11-8.
  2. Jung, D.H. et al. (2006) Biochem Biophys Res Commun 343, 34-42.
  3. Yabe, D. et al. (1997) J Biol Chem 272, 18232-9.
  4. Vorum, H. et al. (1999) Exp Cell Res 248, 473-81.
  5. Vorum, H. et al. (2000) FEBS Lett 465, 129-34.
  6. Teng, L. et al. (2012) PLoS One 7, e40173.
  7. Voisin, S.N. et al. (2011) PLoS One 6, e16352.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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