Product Pathways - Cytoskeletal Signaling
Phospho-KIF1B (Ser1487) Antibody #11993
PhosphoSitePlus® protein, site, and accession data: KIF1B
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H (M) (R) (Mk) | Endogenous | 140, 200 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 11993:
- Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Phospho-KIF1B (Ser1487) Antibody recognizes endogenous levels of KIF1B protein only when phosphorylated at Ser1487.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1487 of human KIF1B protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC-3 cells, treated with Calyculin A #9902 (100 nM, 5 min) or Calyculin A and λ phosphatase, using Phospho-KIF1B (Ser1487) Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Background
Kinesin-like protein KIF1B is a member of the kinesin 3 family of C-kinesins that are characterized by a kinesin-motor domain in the carboxy-terminal region. As part of the general mechanism of kinesin-mediated cellular transport, C-kinesins are known to drive microtubule plus and minus end motilities (1-3). KIF1B is implicated in the transport of synaptic proteins to the cell periphery in neuronal cell axons by interaction with Rab3 guanine nucleotide exchange factor (3). Mitochondria are also often transported in axons by KIF1B (3-4).
Quantitative mass spectrometry profiling of mitotic phosphorylation revealed putative phosphorylation sites of KIF1B (5). Phospho-KIF1B (Ser1487) Antibody is directed at a site identified by Cell Signaling Technology® (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser1487 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.
- Hirokawa, N. et al. (1998) Curr Opin Cell Biol 10, 60-73.
- Dagenbach, E.M. and Endow, S.A. (2004) J Cell Sci 117, 3-7.
- Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
- Wozniak, M.J. et al. (2005) BMC Cell Biol 6, 35.
- Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
