Product Pathways - Tyrosine Kinase / Adaptors
Phospho-DDR1 (Tyr792) Antibody #11994
PhosphoSitePlus® protein, site, and accession data: DDR1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H (M) (R) | Endogenous | 125 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 11994:
- Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Phospho-DDR1 (Tyr792) Antibody recognizes endogenous levels of DDR1 protein only when phosphorylated at Tyr792. This antibody may cross-reacts with other tyrosine-phosphorylated RTKs.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr792 of human DDR1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from T-47D cells, untreated (-) or treated with collagen (15 μg/ml) for the indicated times, using Phospho-DDR1 (Tyr792) Antibody (upper) or DDR1 (D1G6) XP® Rabbit mAb #5583 (lower).
Background
The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1).
Phosphorylation of DDR1 at Tyr792 was identified at Cell Signaling Technology using PTMScan®, our LC-MS/MS platform for phosphorylation site discovery (3). Tyr792 is located in the activation loop of the DDR1 kinase domain.
- Vogel, W. (1999) FASEB J. 13 Suppl, S77-S82.
- Ferri, N. et al. (2004) Am. J. Pathol. 164, 1575-1585.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 5583 DDR1 (D1G6) XP® Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
