Cell Signaling Technology

Product Pathways - Development

GLI1 (L42B10) Mouse mAb (Sepharose Bead Conjugate) #11995

Applications Reactivity Sensitivity MW (kDa) Isotype
IP H Endogenous 160 Mouse IgG1

Applications Key:  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GLI1 (L42B10) Mouse mAb (Sepharose Bead Conjugate) recognizes endogenous levels of total GLI1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human GLI1 protein.

IP

IP

Immunoprecipitation of GLI1 from RMS-13 cell extracts, using GLI1 (L42B10) Mouse mAb (Sepharose Bead Conjugate) (lane 1) or Mouse IgG (Sepharose Bead Conjugate) #3420 (lane 2). Lane 3 is 10% input. Western blot analysis was performed using GLI1 (L42B10) Mouse mAb #2643.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. GLI1 (L42B10) Mouse mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of GLI1. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GLI1 (L42B10) Mouse mAb #2643.

Background

GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5).

  1. Kinzler, K.W. et al. (1987) Science 236, 70-3.
  2. Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
  3. Kinzler, K.W. et al. (1988) Nature 332, 371-4.
  4. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
  5. McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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