Product Pathways - Metabolism
SDHA (D6J9M) XP® Rabbit mAb #11998
|W IP IHC-P IF-IC||H M R Hm Mk||Endogenous||70||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
* Product-specific protocol.
Specificity / Sensitivity
SDHA (D6J9M) XP® Rabbit mAb recognizes endogenous levels of total SDHA protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly166 of human SDHA protein.
Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb.
Immunoprecipitation of SDHA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SDHA (D6J9M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SDHA (D6J9M) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SDHA (D6J9M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Research studies have shown that defects in SDHA cause complex II deficiency (2). In addition, investigators have observed reduction of SDHA in the striatum of patients with Huntington’s disease (3), and reduction of SDHB, SDHC, and SDHD in paragangliomas and phenochromocytomas (4,5).
- Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
- Bourgeron, T. et al. (1995) Nat Genet 11, 144-9.
- Benchoua, A. et al. (2006) Mol Biol Cell 17, 1652-63.
- Baysal, B.E. et al. (2000) Science 287, 848-51.
- Feichtinger, R.G. et al. (2010) BMC Cancer 10, 149.
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For Research Use Only. Not For Use In Diagnostic Procedures.