Product Pathways - Vesicle Trafficking
Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb #12005
|12005S||100 µl (10 western blots)||---||In Stock||---|
|12005||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||125||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb recognizes endogenous levels of REPS1 protein only when phosphorylated at Ser709.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser709 of human REPS1 protein.
Western blot analysis of extracts from Jurkat cells, untreated (-) or calf intestinal phosphatase (CIP)-treated (+), using Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (upper) or REPS1 (D6F4) Rabbit mAb #6404 (lower).
REPS1 is a RalBP1-associated EH-homology domain containing protein. The sequence of REPS1 has an EH domain, followed by two proline-rich segments, and a C-terminal coiled-coil domain for binding to RalBP1 (1). The EH domain of REPS1 interacts with the NPF motif of Rab11-FIP2, mediates their colocalization to endosome vesicles, and influences EGFR endocytosis (2). The two proline-rich regions of REPS1 are important for binding to the SH3 domain of GRK/GRB2 and further regulate EGFR downstream signaling. The proline-rich regions of REPS1 have also been shown to interact with the SH3 domain of intersectin1 (ITSN1) and contribute to ITSN1/SGIP1/REPS1 complex formation on clathrin-coated pits (3). Three alternatively spliced isoforms of REPS1 have been identified.
Phosphorylation of REPS1 at Ser709 was identified at Cell Signaling Technology using PTMScan® Technology, our LC-MS/MS platform for phosphorylation site discovery (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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