Cell Signaling Technology

Product Pathways - Transcription Factors

FoxK2 Antibody #12008

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 79 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FoxK2 Antibody recognizes endogenous levels of total FoxK2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FoxK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and PANC-1 cells using FoxK2 Antibody.

IP

IP

Immunoprecipitation of FoxK2 from PANC-1 cell extracts using Normal Rabbit IgG #2729 (lane 2) or FoxK2 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FoxK2 Antibody.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4).

FoxK1 and FoxK2 belong to one of the subfamilies of Fox proteins and contain a forkhead-associated (FHA) domain and a conserved forkhead DNA binding domain (5). FoxK2 was initially identified as a regulator of IL-2 transcription (6). Studies have shown that FoxK2 binds to DNA harboring G/T mismatches and might be involved in DNA mismatch repair (7). FoxK2 also mediates the oncogenic function of adenovirus (Adv) oncoprotein E1A and papillomavirus E6 proteins via association with these proteins (8). Moreover, FoxK2 promotes AP-1-mediated transcriptional regulation by enhancing the recruitment of AP-1 to chromatin (9). FoxK2 is phosphorylated at Ser368 and Ser423 in a CDK1-cyclin B-dependent manner and is involved in cell cycle regulation (10).

  1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
  2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
  3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
  4. Benayoun, B.A. et al. (2011) Trends Genet, Epub ahead of print.
  5. Katoh, M. and Katoh, M. (2004) Int J Mol Med 14, 127-32.
  6. Li, C. et al. (1991) Proc Natl Acad Sci U S A 88, 7739-43.
  7. Fujii, Y. and Nakamura, M. (2010) J Biochem 147, 705-9.
  8. Komorek, J. et al. (2010) J Virol 84, 2719-31.
  9. Ji, Z. et al. (2012) Mol Cell Biol 32, 385-98.
  10. Marais, A. et al. (2010) J Biol Chem 285, 35728-39.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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