Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SSRP1 Antibody #12009

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk (Hm) (B) (GP) (Hr) Endogenous 81 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine  GP=Guinea Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SSRP1 Antibody recognizes endogenous levels of total SSRP1 protein. This antibody also cross-reacts with a protein of unknown origin at 150 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu148 of human SSRP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SSRP1 Antibody.

Background

Supressor of Ty-16 (SPT16) and structure-specific recognition protein-1 (SSRP1) are subunits of the facilitates chromatin transcription (FACT) complex that is essential for transcription elongation (1,2). FACT facilitates RNA polymerase-dependent transcription of chromatin templates by destabilizing the nucleosomes within the open reading frames of active genes (3-5). FACT destabilizes the nucleosomes, which would otherwise act as barriers to RNA polymerase transcription activity, by disrupting histone-histone and histone-DNA contacts that lead to the eviction of the histone H2A-H2B dimer (2,3,6). FACT may also function as a histone chaperone to reassemble nucleosomes after RNA polymerase passage (7). In addition to transcription, FACT activity has been shown to have a role in DNA replication in yeast and in DNA repair by contributing to the activation of p53 by CK2 and by facilitating histone H2AX-H2B exchange upon DNA damage (8-10).

  1. Winkler, D.D. and Luger, K. (2011) J Biol Chem 286, 18369-74.
  2. Orphanides, G. et al. (1999) Nature 400, 284-8.
  3. Orphanides, G. et al. (1998) Cell 92, 105-16.
  4. Birch, J.L. et al. (2009) EMBO J 28, 854-65.
  5. Orphanides, G. and Reinberg, D. (2000) Nature 407, 471-5.
  6. Keller, D.M. and Lu, H. (2002) J Biol Chem 277, 50206-13.
  7. Belotserkovskaya, R. et al. (2003) Science 301, 1090-3.
  8. Schlesinger, M.B. and Formosa, T. (2000) Genetics 155, 1593-606.
  9. Keller, D.M. and Lu, H. (2002) J Biol Chem 277, 50206-13.
  10. Heo, K. et al. (2008) Mol Cell 30, 86-97.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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