Cell Signaling Technology

Product Pathways - Metabolism

ALDH1A1 (D4R9V) Rabbit mAb #12035

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 55 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ALDH1A1 (D4R9V) Rabbit mAb recognizes endogenous levels of total ALDH1A1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse ALDH1A1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep 3B and Hep G2 cells using ALDH1A1 (D4R9V) Rabbit mAb.

Background

The aldehyde dehydrogenase family is a large group of enzymes that oxidize aldehydes formed through metabolic processes to their carboxylic acids (1). ALDH1A1 is a liver cytosolic isoform of acetaldehyde dehydrogenase and is involved in the major pathway of alcohol metabolism along with alcohol dehydrogenase (2). ALDH1A1 is also known as retinal dehydrogenase 1 and is involved in retinol metabolism, converting retinol to retinoic acid (3). Recent studies suggest that control of retinoid signaling through ALDH1A1 may influence hematopoietic stem cell differentiation (4). There has been recent interest in ALDH1 isoforms as predictive biomarkers in disease. Several studies have suggested that ALDH1A1 is a potential marker for cancer stem cells and chemoresistance in several tumor types, such as melanoma (5), lung cancer (6), glioblastoma (7).

  1. Jackson, B. et al. (2011) Hum Genomics 5, 283-303.
  2. Edenberg, H.J. (2007) Alcohol Res Health 30, 5-13.
  3. Duester, G. (2000) Eur J Biochem 267, 4315-24.
  4. Chute, J.P. et al. (2006) Proc Natl Acad Sci U S A 103, 11707-12.
  5. Luo, Y. et al. (2012) Stem Cells 30, 2100-13.
  6. Huang, C.P. et al. (2013) Cancer Lett 328, 144-51.
  7. Schäfer, A. et al. (2012) Neuro Oncol 14, 1452-64.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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