Product Pathways - Translational Control
Upf1 (D15G6) Rabbit mAb #12040
|12040S||100 µl (10 western blots)||---||In Stock||---|
|12040||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||140||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Upf1 (D15G6) Rabbit mAb recognizes endogenous levels of total Upf1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Upf1 protein.
Western blot analysis of extracts from various cell lines using Upf1 (D15G6) Rabbit mAb.
Immunoprecipitation of Upf1 from LN18 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Upf1 (D15G6) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Upf1 (D15G6) Rabbit mAb.
Confocal immunofluorescent anlaysis of Huh7 cells, untreated (left) or heat shock-treated (right), using Upf1 (D15G6) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).
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