Product Pathways - Translational Control
Upf1 (D15G6) Rabbit mAb #12040
PhosphoSitePlus® protein, site, and accession data: RENT1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R Mk | Endogenous | 140 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Upf1 (D15G6) Rabbit mAb recognizes endogenous levels of total Upf1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Upf1 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using Upf1 (D15G6) Rabbit mAb.
IP
Immunoprecipitation of Upf1 from LN18 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Upf1 (D15G6) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Upf1 (D15G6) Rabbit mAb.
IF-IC
Confocal immunofluorescent anlaysis of Huh7 cells, untreated (left) or heat shock-treated (right), using Upf1 (D15G6) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).
- Leeds, P. et al. (1991) Genes Dev 5, 2303-14.
- Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.
- Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.
- Isken, O. et al. (2008) Cell 133, 314-27.
- Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
