Cell Signaling Technology

Product Pathways - Metabolism

PANK4 (D8N2C) Rabbit mAb #12055

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Hm Mk Endogenous 82 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PANK4 (D8N2C) Rabbit mAb recognizes endogenous levels of total PANK4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn373 of human PANK4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using PANK4 (D8N2C) Rabbit mAb.

IP

IP

Immunoprecipitation of PANK4 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PANK4 (D8N2C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PANK4 (D8N2C) Rabbit mAb.

Background

Panthothenate kinase (PANK) is an enzyme that is responsible for catalyzing the first step in coenzyme A (CoA) synthesis (1-4). There are four human PANK genes (PANK1-4) (1-4). PANK4 is ubiquitously expressed, but higher expression levels are observed in muscle (1,2). PANK4 expression is elevated in rat skeletal muscle under high glucose conditions (2). There is evidence that rat PANK4 colocalizes with pyruvate kinase M2 (PKM2) in vitro (2). PANK4 may also play a protective role in beta-cell apoptosis by lowering the levels of pro-caspase-9 (3). Research studies have shown that mutations in the PANK2 gene are associated with Neurodegeneration with Brain Iron Accumulation (NBIA), formerly known as Hallervorden-Spatz syndrome (1,4). Expression of hPANK4 in a Drosophila model of NBIA rescues the phenotype with the exception of infertility (4).

  1. Zhou, B. et al. (2001) Nat Genet 28, 345-9.
  2. Li, Y. et al. (2005) Mol Cell Biochem 277, 117-25.
  3. Xiang, R.L. et al. (2007) Cell Res 17, 966-8.
  4. Wu, Z. et al. (2009) Hum Mol Genet 18, 3659-72.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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