Cell Signaling Technology

Product Pathways - Neuroscience

mGluR2 Antibody #12056

Applications Reactivity Sensitivity MW (kDa) Source
W IP M R Endogenous 100, >200 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

mGluR2 recognizes endogenous levels of total mGluR2 protein. This antibody does not cross-react with mGluR3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala839 of mouse mGluR2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing HA-tagged full-length mouse mGluR2 (mGluR2-HA; +) or HA-tagged full-length mouse mGluR3 (mGluR3-HA; +), using mGluR2 Antibody (upper) or HA-Tag (C29F4) Rabbit mAb #3724 (lower). HA-tagged mGluR cDNAs were a gift from Dr. Gonzalez-Maeso (Mount Sinai School of Medicine, NY).

Western Blotting

Western Blotting

Western blot analysis of extracts from the indicated cell lines and tissues using mGluR2 Antibody.

IP

IP

Immunoprecipitation of mGluR2 from mouse brain extracts using Normal Rabbit IgG #2729 (lane 2) or mGluR2 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using mGluR2 Antibody.


Background

Metabotropic glutamate receptor 2 (mGluR2) is a class C G protein-coupled receptor for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). While mGluR1 and mGluR5 activate phospholipase C, mGluR2, mGluR3, mGluR4, and mGluR6 are coupled to the inhibitory G protein Gα(i/o) and inhibit adenylyl cyclase (AC) activity (1). Research studies have suggested that mGluR2/3 receptors may be potential targets for the treatment of Schizophrenia (2). Furthermore, mGluR2 interacts with the 5HT2A serotonin receptor to form a hetero-complex in the brain. This complex is a potential pharmacological target for hallucinogenic drugs (3,4).

  1. Pin, J.P. et al. (1994) EMBO J 13, 342-8.
  2. Patil, S.T. et al. (2007) Nat Med 13, 1102-7.
  3. González-Maeso, J. et al. (2008) Nature 452, 93-7.
  4. González-Maeso, J. and Sealfon, S.C. (2009) Trends Neurosci 32, 225-32.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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