Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Tip60 Antibody #12058

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) (Mk) (B) (GP) (Hr) Transfected Only 60 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey  B=Bovine  GP=Guinea Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Tip60 Antibody recognizes transfected levels of total Tip60 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His60 of human Tip60 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with a construct expressing full-length human Tip60 (hTip60; +), using Tip60 Antibody.

Background

Tip60 is a member of the MYST (MOZ, YBF2, SAS2 and Tip60) family of histone acetyltransferases and plays a role in a variety of cellular processes such as transcriptional regulation, DNA repair, and apoptosis (1,2). Tip60 exists as part of a multi-subunit complex that includes proteins such as TRRAP, p400, Reptin, and Pontin (3,4). Tip60 plays important roles in double-stranded DNA break (DSB) repair. Tip60 is required for the activation of the ATM kinase in response to DSBs, as well as acetylation of histones H4 and H2A.X at DSBs to facilitate DNA repair (1,2,5-7). In addition, Tip60 dependent acetylation at Lys120 of p53 within the DNA binding domain is required for the induction of apoptosis upon DNA damage (8,9). Tip60 is involved in a number of transcriptional regulation pathways driven by factors such as nuclear receptors and β-catenin (10-13). The Tip60 complex has been shown to be important for mouse embryonic stem cell self-renewal by regulating transcription of developmental regulators that are controlled by Nanog (14). GSK3 (glycogen synthase kinase-3) mediated phosphorylation at Ser86 of Tip60 promotes Tip60 acetylation and subsequent stimulation of the required autophagy protein ULK1 (15).

  1. Sun, Y. et al. (2010) Cell Cycle 9, 930-6.
  2. Fischle, W. (2009) Nat Cell Biol 11, 1279-81.
  3. Cai, Y. et al. (2003) J Biol Chem 278, 42733-6.
  4. Cai, Y. et al. (2005) J Biol Chem 280, 13665-70.
  5. Sun, Y. et al. (2005) Proc Natl Acad Sci U S A 102, 13182-7.
  6. Murr, R. et al. (2006) Nat Cell Biol 8, 91-9.
  7. Ikura, T. et al. (2007) Mol Cell Biol 27, 7028-40.
  8. Tang, Y. et al. (2006) Mol Cell 24, 827-39.
  9. Sykes, S.M. et al. (2006) Mol Cell 24, 841-51.
  10. Gaughan, L. et al. (2002) J Biol Chem 277, 25904-13.
  11. Jeong, K.W. et al. (2011) Nat Struct Mol Biol 18, 1358-65.
  12. Brady, M.E. et al. (1999) J Biol Chem 274, 17599-604.
  13. Kim, J.H. et al. (2005) Nature 434, 921-6.
  14. Fazzio, T.G. et al. (2008) Cell 134, 162-74.
  15. Lin, S.Y. et al. (2012) Science 336, 477-81.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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