Product Pathways - Translational Control
Exportin 5 Antibody #12068
|W||H (Hm) (Mk) (B) (Pg) (Hr)||Endogenous||136||Rabbit|
Reactivity Key: H=Human Hm=Hamster Mk=Monkey B=Bovine Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Exportin 5 Antibody recognizes endogenous levels of total exportin 5 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu182 of human exportin 5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Import and export through the nuclear envelope (NE) via facilitated translocation is important for many cellular processes including protein synthesis and miRNA biogenesis (1). Exportin 5 (XPO5) is a member of the importin β family of proteins (2) and functions in tRNA export in a sequence dependent fashion. More recently, it has been shown to export pre-miRNA by a RanGTPase-driven process from the nucleus to the cytoplasm, where pre-miRNA processing occurs to produce mature miRNAs (1,3). Study of the miRNA biosynthesis pathway is essential toward understanding the process of oncogenesis as global downregulation of miRNAs and the resulting alterations in expression of tumor suppressor and oncogenic proteins is a common phenotype of cancers cells (3,4). Research studies have shown that disruption of exportin 5 functions in many types of cancers including breast and lung, where pre-miRNA accumulates in the nucleus and miRNA maturation is impaired (2,3).
- Bohnsack, M.T. et al. (2004) RNA 10, 185-91.
- Leaderer, D. et al. (2011) Int J Mol Epidemiol Genet 2, 9-18.
- Melo, S.A. and Esteller, M. (2011) Cell Cycle 10, 922-5.
- Melo, S.A. et al. (2010) Cancer Cell 18, 303-15.
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For Research Use Only. Not For Use In Diagnostic Procedures.