Product Pathways - Translational Control
Exportin 5 Antibody #12068
PhosphoSitePlus® protein, site, and accession data: exportin 5
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (Hm) (Mk) (B) (Pg) (Hr) | Endogenous | 136 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Hm=Hamster
Mk=Monkey
B=Bovine
Pg=Pig
Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 12068:
- Western Blotting
Specificity / Sensitivity
Exportin 5 Antibody recognizes endogenous levels of total exportin 5 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu182 of human exportin 5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using Exportin 5 Antibody.
Background
Import and export through the nuclear envelope (NE) via facilitated translocation is important for many cellular processes including protein synthesis and miRNA biogenesis (1). Exportin 5 (XPO5) is a member of the importin β family of proteins (2) and functions in tRNA export in a sequence dependent fashion. More recently, it has been shown to export pre-miRNA by a RanGTPase-driven process from the nucleus to the cytoplasm, where pre-miRNA processing occurs to produce mature miRNAs (1,3). Study of the miRNA biosynthesis pathway is essential toward understanding the process of oncogenesis as global downregulation of miRNAs and the resulting alterations in expression of tumor suppressor and oncogenic proteins is a common phenotype of cancers cells (3,4). Research studies have shown that disruption of exportin 5 functions in many types of cancers including breast and lung, where pre-miRNA accumulates in the nucleus and miRNA maturation is impaired (2,3).
- Bohnsack, M.T. et al. (2004) RNA 10, 185-91.
- Leaderer, D. et al. (2011) Int J Mol Epidemiol Genet 2, 9-18.
- Melo, S.A. and Esteller, M. (2011) Cell Cycle 10, 922-5.
- Melo, S.A. et al. (2010) Cancer Cell 18, 303-15.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.
