Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

SLBP Antibody #12087

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Dg (Mk) Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SLBP Antibody recognizes endogenous levels of total SLBP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg160 of human SLBP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, asynchronous (-) or synchronized in early S phase by double thymidine block (+), using SLBP Antibody (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

Stem-loop binding protein (SLBP) is the mammalian orthologue of Xenopus SLBP1, a post-transcriptional regulator of histone mRNA. SLBP binds the conserved stem-loop structure at the 3’ end of replication-dependent histone mRNAs. SLBP binding aids in the 3’ end processing of histone pre-mRNA in association with U7 snRNP and the zinc finger protein hZFP100 (1,2), as well as translation and transport of histone mRNA (3).The expression of replication-dependent histone genes peaks during S phase of the cell cycle. Mammalian SLBP is also cell cycle regulated in most somatic cells, but not in mouse oocytes or embryos, indicating a potential role for SLBP in chromatin organization and remodeling (4).Phosphorylation of SLBP at Thr61 by CDK1 regulates SLBP stability by targeting the protein for degradation upon completion of DNA synthesis (5). SLBP association with histone mRNA maintains its stability, and is regulated by the peptidyl-prolyl isomerase, Pin1, and PP2A phosphatase (6,7).

  1. Dominski, Z. et al. (2002) Genes Dev 16, 58-71.
  2. Dominski, Z. et al. (2001) Mol Cell Biol 21, 2008-17.
  3. Sànchez, R. and Marzluff, W.F. (2002) Mol Cell Biol 22, 7093-104.
  4. Allard, P. et al. (2002) J Cell Sci 115, 4577-86.
  5. Koseoglu, M.M. et al. (2008) Mol Cell Biol 28, 4469-79.
  6. Zhang, M. et al. (2012) Biochemistry 51, 3215-31.
  7. Krishnan, N. et al. (2012) Mol Cell Biol 32, 4306-22.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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