Product Pathways - Nuclear Receptor Signaling
PHB2 Antibody #12101
|12101S||100 µl (10 western blots)||---||In Stock||---|
|12101||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||33||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
PHB2 Antibody recognizes endogenous levels of total PHB2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu12 of human PHB2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in nuclear and mitochondrial compartments in eukaryotic cells and exert multiple cellular functions (1). PHB2 (REA) was originally identified as an estrogen receptor (ER) specific coregulator. It directly interacts with hormone-bound ER and represses its transcriptional activity by competitive inhibition of SRC-1 coactivation of ER (2,3). Together with COUP-TFs, PHB2 also interacts with histone deacetylases HDAC1 and HDAC5, and mediates transcriptional regulation by ER through coupling the deacetylase to the transcription activation complex (4). Another important function of PHB2 is stablization of mitochondrial proteins. PHB1/PHB2 hetrodimers form large ring complexes on the mitochondrial membrane (5), acting as chaperones to stablize important mitochodrial proteins, such as OPA1 and Hax1 to support mitochondrial morphogenesis and protection against apoptosis (6-8).
- Mishra, S. et al. J Cell Mol Med 10, 353-63.
- Montano, M.M. et al. (1999) Proc Natl Acad Sci U S A 96, 6947-52.
- Delage-Mourroux, R. et al. (2000) J Biol Chem 275, 35848-56.
- Kurtev, V. et al. (2004) J Biol Chem 279, 24834-43.
- Tatsuta, T. et al. (2005) Mol Biol Cell 16, 248-59.
- Nijtmans, L.G. et al. (2000) EMBO J 19, 2444-51.
- Merkwirth, C. et al. (2008) Genes Dev 22, 476-88.
- Kasashima, K. et al. (2006) J Biol Chem 281, 36401-10.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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