Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

SGK1 (D27C11) Rabbit mAb #12103

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 45-60 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SGK1 (D27C11) Rabbit mAb recognizes endogenous levels of total SGK1 protein. This antibody does not cross-react with SGK2 or SGK3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human SGK1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-MEL-28, SK-MEL-5, and HSG cells using SGK1 (D27C11) Rabbit mAb.

IP

IP

Immunoprecipitation of SGK1 from SK-MEL-28 cell extracts, using Normal Rabbit IgG #2729 (lane 2) or SGK1 (D27C11) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SGK1 (D27C11) Rabbit mAb.

Background

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).

In addition to its membrane channel and carrier functions, SGK1 also regulates a broad range of targets, such as GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, and FKHR-L1 (8,9).

  1. Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
  2. Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
  3. Park, J. et al. (1999) EMBO J 18, 3024-33.
  4. Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
  5. Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
  6. Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
  7. Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.
  8. Lang, F. et al. (2009) Expert Opin Ther Targets 13, 1303-11.
  9. Lang, F. et al. (2010) J Physiol 588, 3349-54.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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