Product Pathways - Nuclear Receptor Signaling
PhosphoPlus® Estrogen Receptor α (Ser167) Antibody Duet #12108
| Duet Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb #5587 | 100 µl | W | H (Mk) | 66 | Rabbit IgG |
| Estrogen Receptor α (D8H8) Rabbit mAb #8644 | 100 µl | W IP IF-IC ChIP | H | 66 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
ChIP=Chromatin IP
Reactivity Key:
H=Human
Mk=Monkey
Species in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5587:
- Western Blotting
- 8644:
- ChIP Agarose, ChIP Magnetic, Immunofluorescence, Immunoprecipitation, Western Blotting
Description
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Background
Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2).Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4).
- Mangelsdorf, D.J. et al. (1995) Cell 83, 835-839.
- Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev. 14, 121-141.
- Chen, D. et al. (1999) Mol. Cell. Biol. 19, 1002-1015.
- Campbell, R.A. et al. (2001) J. Biol. Chem. 276, 9817-9824.
- Chen, D. et al. (2000) Mol. Cell 6, 127-137.
- Joel, P.B. et al. (1998) Mol. Cell. Biol. 18, 1978-1984.
For Research Use Only. Not For Use In Diagnostic Procedures.
