Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-MERIT40 (Ser29) Antibody #12110

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 40 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-MERIT40 (Ser29) Antibody recognizes endogenous levels of MERIT40 protein only when phosphorylated at Ser29.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser29 of human MERIT40 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-MERIT40 (Ser29) Antibody (upper) or MERIT40 Antibody #9500 (lower).

Background

The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of DNA damage response and DNA repair. BRCA1 forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for the regulation of BRCA1 function. The BRCA1-Rap80 complex (BRCA1 A complex), including Rap80, BRCC36, BRCC45, Abraxas, and MERIT40/NBA1, functions in G2/M phase checkpoint control (reviewed in 1,2).MERIT40/NBA1 localizes to sites of DNA damage and is required for the appropriate localization of BRCA1 in response to ionizing radiation, as well as maintenance of the BRCA1 A complex (3,4). Proteomics studies have identified Ser29 as a phosphorylated site on MERIT40/NBA1, and the significance of this phosphorylation is under investigation (5-9).

  1. Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
  2. Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
  3. Wang, B. et al. (2009) Genes Dev 23, 729-39.
  4. Shao, G. et al. (2009) Genes Dev 23, 740-54.
  5. Moritz, A. et al. (2010) Sci Signal 3, ra64.
  6. Rigbolt, K.T. et al. (2011) Sci Signal 4, rs3.
  7. Iliuk, A.B. et al. (2010) Mol Cell Proteomics 9, 2162-72.
  8. Wu, F. et al. (2010) Mol Cell Proteomics 9, 1616-32.
  9. Mayya, V. et al. (2009) Sci Signal 2, ra46.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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