Cell Signaling Technology

Product Pathways - Neuroscience

Brn2/POU3F2 (D2C1L) Rabbit mAb #12137

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-F ChIP H M R Endogenous 55 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Brn2/POU3F2 (D2C1L) Rabbit mAb recognizes endogenous levels of total Brn2/POU3F2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Brn2/POU3F2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Brn2/POU3F2 (D2C1L) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 A-375 cells and either 10 μl of Brn2/POU3F2 (D2C1L) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human MITF Promoter Primers #12377 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

Brn2/POU3F2 is a POU domain-containing transcription factor involved in neuronal differentiation and activation of the corticotrophin-releasing hormone gene (1,2). In mice, disruption of the Brn2 gene results in loss of specific neuronal lineages in the hypothalamus (3). In addition to its role in mammalian neurogenesis, Brn2 has also been implicated in melanoma tumorigenesis and has been shown in the literature to be overexpressed in human melanoma cells compared to normal melanocytes (4,5). Recent studies also identify Brn2 as a transcription factor playing an important role in keratinocyte differentiation (6). Recent reports demonstrate that overexpression of three transcription factors (Brn2, Ascl1, and Myt1L) can directly convert human fibroblasts into functional neurons under precisely defined conditions (7,8).

  1. Fujii, H. and Hamada, H. (1993) Neuron 11, 1197-206.
  2. Schonemann, M.D. et al. (1995) Genes Dev 9, 3122-35.
  3. Nakai, S. et al. (1995) Genes Dev 9, 3109-21.
  4. Cook, A.L. et al. (2003) J Invest Dermatol 121, 1150-9.
  5. Cook, A.L. and Sturm, R.A. (2008) Pigment Cell Melanoma Res 21, 611-26.
  6. Shi, G. et al. (2010) PLoS One 5, e13216.
  7. Pfisterer, U. et al. (2011) Proc Natl Acad Sci USA 108, 10343-8.
  8. Ambasudhan, R. et al. (2011) Cell Stem Cell 9, 113-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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