Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CP110 Antibody #12140

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 130 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CP110 Antibody recognizes endogenous levels of total CP110 protein. This antibody cross-reacts with a protein of unknown origin at ~65 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser580 of human CP110 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cells lines using CP110 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from T98G cells, serum starved for 48 hr and then either untreated (-) or treated with 10% serum for 24 hr (+), using CP110 Antibody (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Background

The centrosome is an organelle that plays an important role in the mammalian cell cycle. Prior to the onset of mitosis, the single interphase centrosome duplicates only once, creating a pair of daughter centrosomes that will form the two spindle poles after breakdown of the nuclear envelope. Failure to duplicate or overduplication of the centrosome can result in polyploidy and genomic instability (reviewed in 1). Centrosomal protein of 110 kDa (CP110) is a cyclin-dependent kinase (CDK) substrate that plays a critical role in promoting the duplication of centrosomes and correct spindle formation (2). In addition, CP110 has been shown to interact with calmodulin (CaM) and centrin to regulate the progression through cytokinesis (3), and with Cep97 and Cep290 to regulate the formation of primary cilia (4,5). CP110 expression is induced in G1/S with peak expression during S-phase. Degradation of CP110 is mediated by cyclin F in G2-phase and is required for normal progression into M-phase (6).

  1. Hinchcliffe, E.H. and Sluder, G. (2001) Genes Dev 15, 1167-81.
  2. Chen, Z. et al. (2002) Dev Cell 3, 339-50.
  3. Tsang, W.Y. et al. (2006) Mol Biol Cell 17, 3423-34.
  4. Spektor, A. et al. (2007) Cell 130, 678-90.
  5. Tsang, W.Y. et al. (2008) Dev Cell 15, 187-97.
  6. D'Angiolella, V. et al. (2010) Nature 466, 138-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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