Product Pathways - Cytoskeletal Signaling
Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb #12152
|12152S||100 µl (10 western blots)||---||In Stock||---|
|12152||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Rat||Endogenous||52||Mouse IgG2b|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Mouse.
Specificity / Sensitivity
Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic acetylpeptide corresponding to residues surrounding Lys40 of human α-tubulin protein.
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
The Elongator complex catalytic subunit (Elp3) acetylates α-tubulin at Lys40, while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-translational modification may be required for dynamic cell shape remodeling, cell motility, tubulin stability, and terminal branching of cortical neurons (2,3).
- Pan, N. et al. (2012) PLoS One 7, e30358. Applications: IF-F.
- Xu, X. et al. (2011) PLoS One 6, e17138. Applications: Western Blotting.
- Piperno, G. and Fuller, M.T. (1985) J Cell Biol 101, 2085-94. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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