Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb #12152

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H R (M) Endogenous 52 Mouse IgG2b

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic acetylpeptide corresponding to residues surrounding Lys40 of human α-tubulin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with TSA #9950 (400 nM, 16 hr; +), using Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

The Elongator complex catalytic subunit (Elp3) acetylates α-tubulin at Lys40, while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-translational modification may be required for dynamic cell shape remodeling, cell motility, tubulin stability, and terminal branching of cortical neurons (2,3).

  1. Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell Biol. 4, 938 -947.
  2. Creppe, C. et al. (2009) Cell 136, 551-64.
  3. Hubbert, C. et al. (2002) Nature 417, 455-8.

Application References

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