Product Pathways - Cell Cycle / Checkpoint
Ki-67 (D3B5) Rabbit mAb (PE Conjugate) #12160
PhosphoSitePlus® protein, site, and accession data: KI-67
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| F | H M R | Endogenous | Rabbit IgG |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 12160:
- Flow
Specificity / Sensitivity
Ki-67 (D3B5) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Ki-67 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human Ki-67 protein.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb (PE Conjugate) and DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.
Background
Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).
- Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
- Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
- Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
- Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9129 Ki-67 (D3B5) Rabbit mAb
- 5742 Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate)
- 8607 PCNA (PC10) Mouse mAb (PE Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
