Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate) #12162

Applications Reactivity Sensitivity MW (kDa) Isotype
IF-IC F H Endogenous 80 Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total HS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro310 of human HS1 protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 (blue) and Jurkat (green) cells using HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of Jurkat (left) and 293 (right) cells using HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate) (red). Blue pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct immunofluorescence and flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated HS1 (D83A8) XP® Rabbit mAb (Human Specific) #3890.

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).

  1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
  2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
  4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
  5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
  6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
  7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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