Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Protein Folding

HSP60 (D6F1) XP® Rabbit mAb #12165

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC F H M R Hm Mk X Z B Pg (C) (Dg) (Hr) Endogenous 60 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  X=Xenopus  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HSP60 (D6F1) XP® Rabbit mAb recognizes endogenous levels of total HSP60 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp68 of human HSP60 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HSP60 (D6F1) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using HSP60 (D6F1) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HSP60 (D6F1) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HSP60 (D6F1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using HSP60 (D6F1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-204 cells using HSP60 (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).

  1. Hartl, F.U. (1996) Nature 381, 571-579.
  2. Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
  3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
  4. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
  5. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
  6. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
  7. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
  8. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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