Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

BLNK (D8R3G) Rabbit mAb #12168

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Endogenous 68, 70 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BLNK (D8R3G) Rabbit mAb recognizes endogenous levels of total BLNK protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BLNK protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BLNK (D8R3G) Rabbit mAb. B cell specificity of this antibody is demonstrated by the absence of signal in Jurkat cells.

Background

B cell linker protein (BLNK), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts at the interface between BCR-associated Syk and downstream signaling cascades (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its amino terminus and an SH2 domain at its carboxy terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs including Tyr72, Tyr84, Tyr96, and Tyr178 (1). These phosphorylated motifs provide docking sites for signaling molecules, such as Btk, PLCγ, and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways (3,4). Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets, such as HPK1, thereby recruiting them to the BCR complex for signaling (5).

  1. Kurosaki, T. and Tsukada, S. (2000) Immunity 12, 1-5.
  2. Fu, C. et al. (1998) Immunity 9, 93-103.
  3. Ishiai, M. et al. (1999) Immunity 10, 117-125.
  4. Baba, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 2582-2586.
  5. Tsuji, S. et al. (2001) J. Exp. Med. 194, 529-539.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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