Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MORF4L1/MRG15 Antibody #12169

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Hm) (B) (Dg) (GP) (Hr) Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  B=Bovine  Dg=Dog  GP=Guinea Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MORF4L1/MRG15 Antibody recognizes endogenous levels of total MORF4L1/MRG15 protein. This antibody does not cross-react with MORF4L2/MRGX.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala237 of human MORF4L1/MRG15 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Huh7, mES, and KNRK cells using MORF4L1/MRG15 Antibody.

Background

MORF4L1/MRG15 is a chromo domain-containing protein that is part of several histone modifying complexes, including the Tip60 histone acetyltransferase (HAT) complex, histone deacetylase (HDAC) complexes, and the JARID1A and JARID1B histone demethylase complexes (1-6). MORF4L1/MRG15 recognizes di- or trimethylated Lys36 of histone H3 through its chromo domain. This interaction recruits and anchors MORF4L1/MRG15-associated chromatin modifying complexes to target genes for transcriptional regulation (7,8). MORF4L1/MRG15 plays a role in DNA repair as part of the Tip60 HAT complex (9,10). MORF4L1/MRG15 regulates alternative splicing during co-transcriptional splicing of mRNA as a part of the JARID1A complex (11). MORF4L1/MRG15 recruitment of the JARID1B complex to embryonic stem cell renewal-associated genes is important for repression of cryptic transcription and maintainance of proper transcriptional elongation (6).

  1. Pardo, P.S. et al. (2002) J Biol Chem 277, 50860-6.
  2. Yochum, G.S. and Ayer, D.E. (2002) Mol Cell Biol 22, 7868-76.
  3. Cai, Y. et al. (2003) J Biol Chem 278, 42733-6.
  4. Doyon, Y. et al. (2004) Mol Cell Biol 24, 1884-96.
  5. Hayakawa, T. et al. (2007) Genes Cells 12, 811-26.
  6. Xie, L. et al. (2011) EMBO J 30, 1473-84.
  7. Zhang, P. et al. (2006) Nucleic Acids Res 34, 6621-8.
  8. Joshi, A.A. and Struhl, K. (2005) Mol Cell 20, 971-8.
  9. Murr, R. et al. (2006) Nat Cell Biol 8, 91-9.
  10. Garcia, S.N. et al. (2007) FEBS Lett 581, 5275-81.
  11. Luco, R.F. et al. (2010) Science 327, 996-1000.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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