Cell Signaling Technology

Product Pathways - Development

KLF4 (D1F2) Rabbit mAb #12173

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H (Mk) Endogenous 62 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

KLF4 (D1F2) Rabbit mAb recognizes endogenous levels of total KLF4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KLF protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KLF4 (D1F2) Rabbit mAb.

Background

KLF4 is a member of the erythroid Kruppel-like factor (EKLF) multigene family that is highly expressed in the differentiating layers of the epidermis (1, 2). KLF4 plays a critical role in the differentiation of epithelial cells and is essential for normal gastric homeostasis (2,3). Depending on the target gene, KLF4 can function as both a repressor and activator of transcription (4). Research studies suggest this protein may function as either a tumor suppressor or an oncogene depending on tumor type, with up-regulation in human squamous cell carcinoma of the head and neck and down-regulation in colorectal carcinoma (5,6). The in vitro reprogramming of somatic cells to an embryonic-like state has been achieved by retroviral transduction of four factors: Oct-3/4, Sox2, c-Myc, and KLF4 (7). These induced pluripotent stem cells (iPS) are of great therapeutic interest as they exhibit the key characteristics and growth properties of pluripotent stem cells (8,9).

  1. Yet, S.F. et al. (1998) J Biol Chem 273, 1026-31.
  2. Segre, J.A. et al. (1999) Nat Genet 22, 356-60.
  3. Katz, J.P. et al. (2005) Gastroenterology 128, 935-45.
  4. Evans, P.M. and Liu, C. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 554-64.
  5. Foster, K.W. et al. (2005) Oncogene 24, 1491-500.
  6. Rowland, B.D. and Peeper, D.S. (2006) Nat Rev Cancer 6, 11-23.
  7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  8. Meissner, A. et al. (2007) Nat Biotechnol 25, 1177-81.
  9. Park, I.H. et al. (2008) Nature 451, 141-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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