Product Pathways - Translational Control
PhosphoPlus® PERK (Thr980) Antibody Duet #12185
|Duet Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179||100 µl||W||R (M)||170||Rabbit IgG|
|PERK (C33E10) Rabbit mAb #3192||100 µl||W||H M R Mk||140||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species in parentheses are predicted to react based on 100% sequence homology.
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.
- Harding, H. et al. (1999) Nature 397, 271-274.
- Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
- Harding, H. et al. (2000) Mol. Cell 5, 897-904.
- Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.
For Research Use Only. Not For Use In Diagnostic Procedures.