Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CCR2 (D14H7) Rabbit mAb #12199

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 42 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CCR2 (D14H7) Rabbit mAb recognizes endogenous levels of total CCR2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly197 of human CCR2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CCR2 (D14H7) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). HEL cells are reported to be negative for CCR2 expression (2).

IP

IP

Immunoprecipitation of CCR2 from THP-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CCR2 (D14H7) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CCR2 (D14H7) Rabbit mAb.

Background

CCR2 is a member of the “CC-branch” of chemokine G protein-coupled receptors that regulate monocyte chemotaxis and T cell migration/activation and drive inflammation in a number of pathological conditions (1). CCR2 is the receptor for several chemokines including MCP-1, MCP-3, and MCP-4 (2-5). CCR2 transduces signals through increases in intracellular calcium levels. It has two alternative isoforms, CCR2A and CCR2B, differing in their carboxy-terminal tails with CCR2B trafficking more efficiently to the membrane (2,6). CCR2 was originally identified in the THP-1 monocyte cell line, and its expression is decreased following differentiation into macrophages (7). Knockout studies demonstrate that CCR2 is a major regulator of macrophage trafficking (8-10). In addition, research studies have shown that CCR2 functions as an alternative coreceptor with CD4 for infection of some strains of HIV (11,12).

  1. Zhao, Q. (2010) J Leukoc Biol 88, 41-55.
  2. Charo, I.F. et al. (1994) Proc Natl Acad Sci U S A 91, 2752-6.
  3. Franci, C. et al. (1995) J Immunol 154, 6511-7.
  4. Yamagami, S. et al. (1994) Biochem Biophys Res Commun 202, 1156-62.
  5. Combadiere, C. et al. (1995) J Biol Chem 270, 29671-5.
  6. Wong, L.M. et al. (1997) J Biol Chem 272, 1038-45.
  7. Denholm, E.M. and Stankus, G.P. (1995) Cytokine 7, 436-40.
  8. Kuziel, W.A. et al. (1997) Proc Natl Acad Sci U S A 94, 12053-8.
  9. Boring, L. et al. (1997) J Clin Invest 100, 2552-61.
  10. Kurihara, T. et al. (1997) J Exp Med 186, 1757-62.
  11. Rucker, J. et al. (1996) Cell 87, 437-46.
  12. Frade, J.M. et al. (1997) J Clin Invest 100, 497-502.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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