Cell Signaling Technology

Product Pathways - Protein Folding

FKBP5 (D5G2) Rabbit mAb #12210

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 51 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FKBP5 (D5G2) Rabbit mAb recognizes endogenous levels of total FKBP5 protein. This antibody does not cross-react with FKBP4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg222 of human FKBP5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080 and HeLa cells using FKBP5 (D5G2) Rabbit mAb.

IP

IP

Immunoprecipitation of FKBP5 from HT-1080 cell extracts, using Normal Rabbit IgG #2729 (lane 2) or FKBP5 (D5G2) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FKBP5 (D5G2) Rabbit mAb.

Background

FK506 binding protein 51 (FKBP51, also called FKBP5) belongs to the FKBP family of immunophilins (1). FKBP family proteins contain FK domains and TPR (tetratricopeptide repeat) domains. The FK domains are responsible for PPIase (peptidylprolyl isomerase) acitivity and allow binding to FK506 and rapamycin (2,3). The C terminal TPR domains are involved in protein-protein interactions. The TPR domain of FKBP5 mediates binding to HSP90 complexes (4), as well as glucocorticoid, androgen, and progesterone receptors, which account for its regulatory role in steroid hormone receptor function (5). FKBP5 also binds to IKKα and is involved in NF-κB signaling (6,7). In addition, FKBP5 was identified as a negative regulator of Akt, through promotion of Akt - PHLPP interaction and enhanced dephosphorylation of Akt (8).

  1. Avilés, A. et al. (1995) Clin Oncol (R Coll Radiol) 7, 97-101.
  2. Fruman, D.A. et al. (1994) FASEB J 8, 391-400.
  3. Fix, M.L. et al. (2008) Blood Press Monit 13, 199-203.
  4. Scheufler, C. et al. (2000) Cell 101, 199-210.
  5. Schülke, J.P. et al. (2010) PLoS One 5, e11717.
  6. Bouwmeester, T. et al. (2004) Nat Cell Biol 6, 97-105.
  7. Avellino, R. et al. (2005) Blood 106, 1400-6.
  8. Pei, H. et al. (2009) Cancer Cell 16, 259-66.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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