Cell Signaling Technology

Product Pathways - Neuroscience

SHANK2 Antibody #12218

Applications Reactivity Sensitivity MW (kDa) Source
W IP M R (H) (Mk) (B) Endogenous 165 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SHANK2 Antibody recognizes endogenous levels of total SHANK2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val537 of human SHANK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western blot analysis of extracts from the indicated rat brain synaptic fractions using SHANK2 Antibody. Separation of the different synaptic fractions was confirmed using PSD95 (D27E11) XP® Rabbit mAb #3450 and Syntaxin 6 (C34B2) Rabbit mAb #2869. Equal loading of each fraction was assessed using β-Tubulin (9F3) Rabbit mAb #2128. Fractionation of the different synaptic compartments was carried out as described by Phillips, G.R. et al. (2001) Neuron 32, 63-77. PAZ, Pre-synaptic active zone.

Western blot analysis of extracts from mouse and rat brain tissue using SHANK2 Antibody.

Background

The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (6).

  1. Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.
  2. Boeckers, T.M. et al. (1999) J Neurosci 19, 6506-18.
  3. Boeckers, T.M. et al. (2005) J Neurochem 92, 519-24.
  4. Sala, C. et al. (2001) Neuron 31, 115-30.
  5. Boeckers, T.M. et al. (2002) J Neurochem 81, 903-10.
  6. Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products