Product Pathways - Neuroscience
Tenascin C (D16C4) Rabbit mAb #12221
PhosphoSitePlus® protein, site, and accession data: TNC
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 240 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 12221:
- Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Tenascin C (D16C4) Rabbit mAb recognizes endogenous levels of total Tenascin C protein. This antibody also cross-reacts with a protein of unknown origin at 120 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Tenascin C protein.
Western Blotting
Western blot analysis of extracts from mouse brain and rat brain using Tenascin C (D16C4) Rabbit mAb.
Background
Tenascin C is a large hexameric extracellular matrix glycoprotein that exhibits de-adhesive effects on cell-matrix interaction, enhancing cell proliferation and motility in most cell types. It is highly expressed in remodeling tissues during embryonic development and under pathological conditions in adults, and research studies have shown markedly increased expression in cancerous tissues (1,2). Tenascin C has been implicated in a variety of cellular processes relevant to atherosclerosis, including cell proliferation, migration, and apoptosis. Expression of Tenascin C is tightly controlled in adults and is upregulated in tissues undergoing wound healing (3). In development, the expression of Tenascin C is known to be associated with epithelial-mesenchymal transition (EMT) events including gastrulation and formation of the neural crest, endocardial cushion, and secondary palate (1). Investigators have shown that Tenascin C is a key determinant of the tumor stroma and that it is involved in the initiation of tumorigenesis and progression to metastasis (2). Immature and mature astrocytes, radial glial cells, Schwann cells, and a subset of neurons express Tenascin C. Upon CNS trauma or exposure of neurons to excitotoxic agents, Tenascin C expression is upregulated by glial cells. Research studies have shown that Tenascin C is involved in guidance of migrating axons and neurons, synaptic plasticity, and neuronal regeneration, promoting spinal cord regeneration after injury (4).
- Imanaka-Yoshida, K. (2012) Circ J 76, 2513-20.
- Yoshimura, H. et al. (2011) Histol Histopathol 26, 297-305.
- Minear, M.A. et al. (2011) Hum Genet 129, 641-54.
- Chen, J. et al. (2010) Mol Ther 18, 1769-77.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
