Product Pathways - Lymphocyte Signaling
FLI1 Antibody #12225
|12225S||100 µl (10 western blots)||---||In Stock||---|
|12225||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||52||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
FLI1 Antibody recognizes endogenous levels of total FLI1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val64 of human FLI1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Friend leukemia integration 1 (FLI1) transcription factor is an ETS domain-containing transcription factor that plays an important and highly conserved role in vertebrate development, particularly hematopoiesis, where it functions to activate transcription of genes that promote erythroblast proliferation (1-4). In mice, the Fli1 locus is a common retroviral insertion site for the Friend Murine Leukemia Virus (F-MuLV), such that a majority of F-MuLV-induced erythroleukemias are associated with aberrant Fli1 expression (5). Notably in humans, aberrant FLI1 expression has also been linked to poor prognosis in acute myeloid leukemia (6). Also in humans, a t(11;22)(q24;q12) chromosomal translocation has been described that generates a chimeric protein (EWS/FLI1) comprised of the amino-terminal transactivation domain of Ewing Sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of FLI1 (7). The EWS/FLI1 fusion protein functions as a transcriptional activator that is reportedly responsible for >85% of the known cases of pediatric Ewing’s Sarcoma, an aggressive bone and soft tissue tumor (8,9).
- Spyropoulos, D.D. et al. (2000) Mol Cell Biol 20, 5643-52.
- Pimanda, J.E. et al. (2007) Proc Natl Acad Sci U S A 104, 17692-7.
- Jagadeeswaran, P. et al. (2010) Blood Cells Mol Dis 44, 175-80.
- Tijssen, M.R. et al. (2011) Dev Cell 20, 597-609.
- Ben-David, Y. et al. (1990) Proc Natl Acad Sci U S A 87, 1332-6.
- Kornblau, S.M. et al. (2011) Blood 118, 5604-12.
- May, W.A. et al. (1993) Proc Natl Acad Sci U S A 90, 5752-6.
- Braun, B.S. et al. (1995) Mol Cell Biol 15, 4623-30.
- Riggi, N. and Stamenkovic, I. (2007) Cancer Lett 254, 1-10.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.