Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

FLI1 Antibody #12225

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 52 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FLI1 Antibody recognizes endogenous levels of total FLI1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val64 of human FLI1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FLI1 Antibody.

Background

Friend leukemia integration 1 (FLI1) transcription factor is an ETS domain-containing transcription factor that plays an important and highly conserved role in vertebrate development, particularly hematopoiesis, where it functions to activate transcription of genes that promote erythroblast proliferation (1-4). In mice, the Fli1 locus is a common retroviral insertion site for the Friend Murine Leukemia Virus (F-MuLV), such that a majority of F-MuLV-induced erythroleukemias are associated with aberrant Fli1 expression (5). Notably in humans, aberrant FLI1 expression has also been linked to poor prognosis in acute myeloid leukemia (6). Also in humans, a t(11;22)(q24;q12) chromosomal translocation has been described that generates a chimeric protein (EWS/FLI1) comprised of the amino-terminal transactivation domain of Ewing Sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of FLI1 (7). The EWS/FLI1 fusion protein functions as a transcriptional activator that is reportedly responsible for >85% of the known cases of pediatric Ewing’s Sarcoma, an aggressive bone and soft tissue tumor (8,9).

  1. Spyropoulos, D.D. et al. (2000) Mol Cell Biol 20, 5643-52.
  2. Pimanda, J.E. et al. (2007) Proc Natl Acad Sci U S A 104, 17692-7.
  3. Jagadeeswaran, P. et al. (2010) Blood Cells Mol Dis 44, 175-80.
  4. Tijssen, M.R. et al. (2011) Dev Cell 20, 597-609.
  5. Ben-David, Y. et al. (1990) Proc Natl Acad Sci U S A 87, 1332-6.
  6. Kornblau, S.M. et al. (2011) Blood 118, 5604-12.
  7. May, W.A. et al. (1993) Proc Natl Acad Sci U S A 90, 5752-6.
  8. Braun, B.S. et al. (1995) Mol Cell Biol 15, 4623-30.
  9. Riggi, N. and Stamenkovic, I. (2007) Cancer Lett 254, 1-10.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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